Hookworms digest blood-derived hemoglobin using a range of mechanistically distinct proteases, and preliminary data have suggested that Ac-CP-2, a cathepsin B cysteine protease from A. caninum, might be involved in this pathway . With the aim of eventually vaccinating people against human hookworm disease, we decided to vaccinate dogs against the canine hookworm A. caninum with catalytically active recombinant Ac-CP-2, to determine whether vaccinated animals were protected against hookworm disease. We show that the cathepsin B-like protease Ac-CP-2 is secreted as a proteolytically active enzyme by the yeast Pichia pastoris and that the enzyme is expressed in the intestinal lumen of blood-feeding adult hookworm parasites. The vaccination of dogs with Ac-CP-2, formulated with several discrete adjuvants, resulted in reduced fecal egg counts and a decreased size of female and male worms. Moreover, the number of female hookworms present in the intestines of vaccinated dogs was significantly lower than that in control dogs. Antibodies generated by vaccinated dogs bound to the intestinal lumen and to intestinal contents of hookworms recovered from those dogs, and they interfered with the proteolytic function of the recombinant Ac--CP-2 enzyme in vitro.
Digestive enzymes are vital for maintaining good health in dogs
Dogs and cats also benefit from the addition of systemic enzymes to their diet for their anti-inflammatory properties. The ingestion of blends of enzymes containing specific proteolytic enzymes such as bromelain are very effective in relieving inflammation thus helps in healing.
Digestive Enzyme Supplements | Whole Dog Journal
Enzymes have been found to be a greatly beneficial ingredient when added to a working dogs nutritional program. Enzymes are natural catalysts which work in the body by hastening the breakdown of the foods which dogs consume, thus maximizing the utilization of the nutrients in the foods. Often working dogs are consuming a unique and controlled diet, high in proteins. These dogs can greatly benefit, both in comfort and in results with added enzymes to break down the high levels of proteins they are consuming. Thus, an addition of enzymes both hastens and maximizes the results of their specialized diets. Dogs such as sled dogs, bomb dogs and rescue dogs also benefit from the addition of extra enzymes to their diet for their anti-inflammatory properties. The ingestion of blends of enzymes containing specific proteolytic enzymes such as bromelain are very effective in relieving inflammation, thus hastening healing.
Prozyme Enzyme Food Supplement for Dogs Cats - Only Natural Pet